Journal: Frontiers in Microbiology

Article Title: Characterizations of bla CTX–M–14 and bla CTX–M–64 in a clinical isolate of Escherichia coli from China

doi: 10.3389/fmicb.2023.1158659

Figure Lengend Snippet: Mics of antibiotics and compounds.

Article Snippet: After 1,000-fold dilution, 100 μL was spread on LB nutrient agar medium containing 100 μg/mL ampicillin (Solarbio Science & Technology, Beijing, China) and 750 μg/mL rifampicin (Solarbio Science & Technology, Beijing, China).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Characterizations of bla CTX–M–14 and bla CTX–M–64 in a clinical isolate of Escherichia coli from China

doi: 10.3389/fmicb.2023.1158659

Figure Lengend Snippet: Relative changes in resistance gene mRNA levels in strains Ec15103, Ec15103-Ec600, and Ec15103-EPI300-1 under the influence of antibiotics. (A,B) Relative changes in bla CTX–M–14 and bla CTX–M–64 mRNA levels in strain Ec15103 under different concentrations of cefotaxime (CTX); bla CTX–M–14 is present on the Ec15103 plasmid pEc15103B and bla CTX–M–64 on the Ec15103 chromosome. (C) Relative changes in bla CTX–M–14 mRNA levels in strain Ec15103-Ec600 at different concentrations of CTX. bla CTX–M–14 is located on the Ec15103-Ec600 conjugate plasmid pEc15103B. (D) Relative changes in bla TEM–1 mRNA levels in strain Ec15103-EPI300-1 at different concentrations of ampicillin (AMP). bla TEM–1 is located on the Ec15103-EPI300-1 transconjugant plasmid pEc15103A. For at least two independent experiments, each with at least three biological replicates, the data shown represent the mean standard deviation using one-way ANOVA.

Article Snippet: After 1,000-fold dilution, 100 μL was spread on LB nutrient agar medium containing 100 μg/mL ampicillin (Solarbio Science & Technology, Beijing, China) and 750 μg/mL rifampicin (Solarbio Science & Technology, Beijing, China).

Techniques: Plasmid Preparation, Standard Deviation